Rhizobium Strain Identification and Quantification in Commercial Inoculants by Immunoblot Analysis
نویسندگان
چکیده
منابع مشابه
Rhizobium strain identification and quantification in commercial inoculants by immunoblot analysis.
An immunoblot procedure for the strain-specific quantitative analysis of commercial Rhizobium inoculants was developed. The technique greatly reduced the time required for inoculant analysis. Correlation between immunoblot analysis and traditional plant nodule grow-out most-probable-number techniques was r = 0.90 for 16 commercial alfalfa inoculants tested.
متن کاملNodule occupancy determination and Rhizobium strain quantification by immunoblot assay
Dot immunablot assay was used for determination of nodules produced on soybean. french bean. pigeon pea and urdbean by Bradyrhizobiumjaponicum USDA-I 10; R. leguminosarum bv. phaseoli FB-77 and N-3; Rhizobium sp. A-3 and U-I. respectively. Nodule occupancy by inoculated strains as determined by the test ranged between 73 to 93%. Replica inmrunoblot assay reduced the time required for enumeratio...
متن کاملIdentification of Rhizobium spp. in peat-based inoculants by DNA hybridization and PCR and its application in inoculant quality control.
Procedures based on DNA hybridization and PCR were developed for quality control of Rhizobium inoculants. Inoculants for pea and goat's rue were produced by Elomestari Ltd., Juva, Finland, in sterile dry fine peat by the standard procedure used by the company. The inoculants contained Rhizobium galegae HAMBI 1174 and HAMBI 1207 and an R. leguminosarum biovar vicia strain, 16HSA, either solely o...
متن کاملSurvival of Rhizobium phaseoli in Coal-Based Legume Inoculants.
The long-term survival of Rhizobium phaseoli strains 127K17, 127K26, and 127K35 in legume inoculants prepared with eight different coals (one strain and one coal per inoculant) was studied. The coals used were Pennsylvania anthracite, bituminous coals from Illinois, Pennsylvania, and Utah, lignite from North Dakota and Texas, and subbituminous coals from New Mexico and Wyoming; they ranged in p...
متن کاملChoosing a Commercial Broiler Strain Based on Multicriteria Decision Analysis
With the complexity and amount of information in a wide variety of comparative performance reports in poultry production, making a decision is difficult. This problem is overcomed only when all data can be put into a common unit. For this purpose, five different decision making analysis approaches including Maximin, Equally likely, Weighted average, Ordered weighted averages and Technique for ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1989
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.55.2.520-522.1989